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Simple method for demonstrating small plasmid deoxyribonucleic acid molecules in oral streptococci.

A simple procedure for rapidly demonstrating small plasmids (less than 10 megadaltons) in oral streptococci is described. Logarithmic-phase, glycine-treated cells from 1.5-ml broth cultures were converted to osmotically fragile forms and lysed with sodium dodecyl sulfate. After the hydrodynamic shea...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Macrina, F L, Wood, P H, Jones, K R
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1980
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC291477/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7396483/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.39.5.1070-1073.1980
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