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Novel Method of Cell-Free In Vitro Synthesis of the Human Fibroblast Growth Factor 1 Gene

Recombinant DNA projects generally involve cell-based gene cloning. However, because template DNA is not always readily available, in vitro chemical synthesis of complete genes from DNA oligonucleotides is becoming the preferred method for cloning. This article describes a new, rapid procedure based...

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Bibliografiske detaljer
Main Authors: Zuo, Peijun, Rabie, A. Bakr M.
Format: Artigo
Sprog:Inglês
Udgivet: Hindawi Publishing Corporation 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2913909/
https://ncbi.nlm.nih.gov/pubmed/20706664
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2010/971340
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