Rapid Membrane Filtration-Epifluorescent Microscopy Technique for Direct Enumeration of Bacteria in Raw Milk

Membrane filtration and epifluorescent microscopy were used for the direct enumeration of bacteria in raw milk. Somatic cells were lysed by treatment with trypsin and Triton X-100 so that 2 ml of milk containing up to 5 × 10(6) somatic cells/ml could be filtered. The majority of the bacteria (ca. 80...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Pettipher, Graham L., Mansell, Roderick, McKinnon, Charles H., Cousins, Christina M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1980
Assuntos:
Food Microbiology and Toxicology
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC291347/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16345515/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.39.2.423-429.1980
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