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Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation

BACKGROUND: The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells...

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Detalhes bibliográficos
Main Authors: Faize, Mohamed, Faize, Lydia, Burgos, Lorenzo
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2912785/
https://ncbi.nlm.nih.gov/pubmed/20637070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-53
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