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Polyethyleneimine-mediated transfection of cultured postmitotic neurons from rat sympathetic ganglia and adult human retina

BACKGROUND: Chemical methods of transfection that have proven successful with cell lines often do not work with primary cultures of neurons. Recent data, however, suggest that linear polymers of the cation polyethyleneimine (PEI) can facilitate the uptake of nucleic acids by neurons. Consequently, w...

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Detalhes bibliográficos
Main Authors: Horbinski, Craig, Stachowiak, Michal K, Higgins, Dennis, Finnegan, Sarah G
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC29102/
https://ncbi.nlm.nih.gov/pubmed/11231879
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2202-2-2
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