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A simple method for generating full length cDNA from low abundance partial genomic clones

BACKGROUND: PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability may not be sufficient to generate a desired target molecule from two separate regions involving intron /exon bounda...

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Publicado no:BMC Mol Biol
Principais autores: Wang, Yongxin, Fugaro, Joseph M, Siddiq, Fauzia, Goparaju, Chandra Mouli V, Lonardo, Fulvio, Wali, Anil, Lechner, John F, Pass, Harvey I
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2000
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29089/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11114844/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2199-1-2
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