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Using fluorometry and ion-sensitive microelectrodes to study the functional expression of heterologously-expressed ion channels and transporters in Xenopus oocytes

The Xenopus laevis oocyte is a model system for the electrophysiological study of exogenous ion transporters. Three main reasons make the oocyte suitable for this purpose: (a) it has a large cell size (~1 mm diameter), (b) it has an established capacity to produce—from microinjected mRNAs or cRNAs—e...

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Autores principales: Musa-Aziz, Raif, Boron, Walter F., Parker, Mark D.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2905798/
https://ncbi.nlm.nih.gov/pubmed/20051266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2009.12.012
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