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Visualizing lens epithelial cell proliferation in whole lenses

PURPOSE: To develop a means to image cells in S-phase of the cell cycle while preserving the anatomic relationships within the lens. METHODS: Mice were injected with the thymidine analog, EdU. Whole lenses were removed, fixed and permeabilized. Cells that had incorporated EdU into their DNA were che...

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Main Authors: Wiley, Luke A., Shui, Ying-Bo, Beebe, David C.
Format: Artigo
Jezik:Inglês
Izdano: Molecular Vision 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2903465/
https://ncbi.nlm.nih.gov/pubmed/20664699
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