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Comparison of virus isolation using the Vero E6 cell line with real-time RT-PCR assay for the detection of human metapneumovirus

BACKGROUND: The use of cell culture for the diagnosis of human metapneumovirus (hMPV) infection is uncommon at present and molecular method such as reverse-transcription PCR (RT-PCR) has been widely and most commonly used as the preferred test. We aimed to compare the results of virus isolation usin...

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Detalhes bibliográficos
Main Authors: Matsuzaki, Yoko, Mizuta, Katsumi, Takashita, Emi, Okamoto, Michiko, Itagaki, Tsutomu, Katsushima, Fumio, Katsushima, Yuriko, Nagai, Yukio, Nishimura, Hidekazu
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2902479/
https://ncbi.nlm.nih.gov/pubmed/20546620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2334-10-170
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