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Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins
BACKGROUND: Technology used for the purification of recombinant proteins is a key issue for the biochemical and structural analyses of proteins. In general, affinity tags, such as glutathione-S-transferase or six-histidines, are used to purify recombinant proteins. Since such affinity tags often int...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2010
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2901362/ https://ncbi.nlm.nih.gov/pubmed/20525307 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-42 |
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