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Photoactivatable mCherry for high-resolution two-color fluorescence microscopy

The reliance of modern microscopy techniques on photoactivatable fluorescent proteins prompted development of mCherry variants that are initially dark but become red fluorescent after violet-light irradiation. Using ensemble and single-molecule characteristics as selection criteria, we developed PAm...

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Detalhes bibliográficos
Main Authors: Subach, Fedor V, Patterson, George H, Manley, Suliana, Gillette, Jennifer M, Lippincott-Schwartz, Jennifer, Verkhusha, Vladislav V
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2901231/
https://ncbi.nlm.nih.gov/pubmed/19169259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1298
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