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Dual Reporter System for In Situ Detection of Plasmid Transfer under Aerobic and Anaerobic Conditions
We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs a...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology (ASM)
2010
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2897451/ https://ncbi.nlm.nih.gov/pubmed/20453134 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00226-10 |
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