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Dual Reporter System for In Situ Detection of Plasmid Transfer under Aerobic and Anaerobic Conditions

We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs a...

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Detalhes bibliográficos
Main Authors: Król, Jaroslaw E., Rogers, Linda M., Krone, Stephen M., Top, Eva M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2897451/
https://ncbi.nlm.nih.gov/pubmed/20453134
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00226-10
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