Pentaplexed Quantitative Real-Time PCR Assay for the Simultaneous Detection and Quantification of Botulinum Neurotoxin-Producing Clostridia in Food and Clinical Samples

Botulinum neurotoxins are produced by the anaerobic bacterium Clostridium botulinum and are divided into seven distinct serotypes (A to G) known to cause botulism in animals and humans. In this study, a multiplexed quantitative real-time PCR assay for the simultaneous detection of the human pathogen...

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Main Authors: Kirchner, Sebastian, Krämer, K. Melanie, Schulze, Martin, Pauly, Diana, Jacob, Daniela, Gessler, Frank, Nitsche, Andreas, Dorner, Brigitte G., Dorner, Martin B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2897425/
https://ncbi.nlm.nih.gov/pubmed/20435756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02490-09
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