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Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum
BACKGROUND: To maintain pluripotency of human embryonic stem (huES) cells in feeder-free culture it has been necessary to provide a Matrigel substratum, which is a complex of poorly defined extracellular matrices and growth factors derived from mouse Engelbreth-Holm-Swarm sarcoma cells. Culture of s...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
BioMed Central
2010
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2896937/ https://ncbi.nlm.nih.gov/pubmed/20525219 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-213X-10-60 |
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