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Visualizing high error levels during gene expression in living bacterial cells
To monitor inaccuracy in gene expression in living cells, we designed an experimental system in the bacterium Bacillus subtilis whereby spontaneous errors can be visualized and quantified at a single-cell level. Our strategy was to introduce mutations into a chromosomally encoded gfp allele, such th...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2010
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2895060/ https://ncbi.nlm.nih.gov/pubmed/20534550 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0912989107 |
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