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Activation of the Phosphosignaling Protein CheY II. Analysis of activated mutants by (19)F NMR and protein engineering

The Escherichia coli CheY protein is activated by phosphorylation, and in turn alters flagellar rotation. To investigate the molecular mechanism of activation, an extensive collection of mutant CheY proteins was analyzed by behavioral assays, in vitro phosphorylation, and (19)F NMR chemical shift me...

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Bibliographic Details
Main Authors: Bourret, Robert B., Drake, Steven K., Chervitz, Stephen A., Simon, Melvin I., Falke, Joseph J.
Format: Artigo
Language:Inglês
Published: 1993
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2892986/
https://ncbi.nlm.nih.gov/pubmed/8514750
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