Recovery of biologically active spleen focus-forming virus from molecularly cloned spleen focus-forming virus-pBR322 circular DNA by cotransfection with infectious type C retroviral DNA.

The genome of the Lilly-Steeves strain of spleen focus-forming virus (SFFV) was molecularly cloned in the plasmid vector pBR322. Infectious SFFV could be recovered by releasing the SFFV DNA from the vector, transfecting the released DNA onto NIH 3T3 cells, and rescuing the SFFV either by superinfect...

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Bibliographic Details
Main Authors: Linemeyer, D L, Ruscetti, S K, Menke, J G, Scolnick, E M
Format: Artigo
Language:Inglês
Published: 1980
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC288865/
https://ncbi.nlm.nih.gov/pubmed/6252333
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