Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29.

We isolated phi 29 DNA replicative intermediates from extracts of phage-infected Bacillus subtilis, pulsed-labeled with [3H]thymidine, by velocity sedimentation in neutral sucrose followed by CsCl equilibrium density gradient centrifugation. During a chase, the DNA with a higher sedimentation coeffi...

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Bibliographic Details
Published in:J Virol
Main Authors: Inciarte, M R, Salas, M, Sogo, J M
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1980
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC288685/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6768899/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.34.1.187-199.1980
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