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A Method for Uptake Quantification of Multiple Fluorescent DNAs in Boar Semen As an Alternative to Radiolabeling
Sperm-mediated gene transfer (SMGT) is a simple and efficient method for producing multitransgenic organisms. Until now, the exogenous DNA uptake efficiencies have been quantified, performing coincubation of spermatozoa with (3)H-DNA. This method has significant limitations; from a researcher's...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Association of Biomolecular Resource Facilities
2010
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2884311/ https://ncbi.nlm.nih.gov/pubmed/20592868 |
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