A Method for Uptake Quantification of Multiple Fluorescent DNAs in Boar Semen As an Alternative to Radiolabeling

Sperm-mediated gene transfer (SMGT) is a simple and efficient method for producing multitransgenic organisms. Until now, the exogenous DNA uptake efficiencies have been quantified, performing coincubation of spermatozoa with (3)H-DNA. This method has significant limitations; from a researcher's...

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Detaylı Bibliyografya
Asıl Yazarlar: De Cecco, Marco, Zannoni, Augusta, Bernardini, Chiara, Lavitrano, Marialuisa, Bacci, Maria Laura, Forni, Monica
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Association of Biomolecular Resource Facilities 2010
Konular:
Communications
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2884311/
https://ncbi.nlm.nih.gov/pubmed/20592868
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