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Blunt-ended DNA double-strand breaks induced by endonucleases PvuII and EcoRV are poor substrates for repair in Saccharomyces cerevisiae

Most mechanistic studies of repair of DNA double-strand breaks (DSBs) produced by in vivo expression of endonucleases have utilized enzymes that produce cohesive-ended DSBs such as HO, I-SceI and EcoRI. We have developed systems for expression of PvuII and EcoRV, nucleases that produce DSBs containi...

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Autores principales: Westmoreland, James W., Summers, Jennifer A., Holland, Cory L., Resnick, Michael A., Lewis, L. Kevin
Formato: Artigo
Lenguaje:Inglês
Publicado: 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2883614/
https://ncbi.nlm.nih.gov/pubmed/20356803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.dnarep.2010.02.008
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