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Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2

Purified thermostable recombinant L2 lipase from Bacillus sp. L2 was crystallized by the counter-diffusion method using 20% PEG 6000, 50 mM MES pH 6.5 and 50 mM NaCl as precipitant. X-ray diffraction data were collected to 2.7 Å resolution using an in-house Bruker X8 PROTEUM single-crystal diffracto...

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Main Authors: Shariff, Fairolniza Mohd, Rahman, Raja Noor Zaliha Raja Abd., Ali, Mohd Shukuri Mohamad, Chor, Adam Leow Thean, Basri, Mahiran, Salleh, Abu Bakar
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2882778/
https://ncbi.nlm.nih.gov/pubmed/20516608
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911001482X
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