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Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA
In trypanosomes a 39 nucleotide exon, the spliced leader (SL) is donated to all mRNAs from a small RNA, the SL RNA, by trans-splicing. Since the discovery of trans-splicing in trypanosomes two decades ago, numerous attempts failed to reconstitute the reaction in vitro. In this study, a crude whole-c...
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| Autors principals: | , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2879531/ https://ncbi.nlm.nih.gov/pubmed/20159996 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq065 |
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