Production of Infectious Hepatitis C Virus by Using RNA Polymerase I-Mediated Transcription

In this study, we used an RNA polymerase I (Pol I) transcription system for development of a reverse genetics protocol to produce hepatitis C virus (HCV), which is an uncapped positive-strand RNA virus. Transfection with a plasmid harboring HCV JFH-1 full-length cDNA flanked by a Pol I promoter and...

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Detalhes bibliográficos
Main Authors: Masaki, Takahiro, Suzuki, Ryosuke, Saeed, Mohsan, Mori, Ken-ichi, Matsuda, Mami, Aizaki, Hideki, Ishii, Koji, Maki, Noboru, Miyamura, Tatsuo, Matsuura, Yoshiharu, Wakita, Takaji, Suzuki, Tetsuro
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Genome Replication and Regulation of Viral Gene Expression
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2876618/
https://ncbi.nlm.nih.gov/pubmed/20237083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.02397-09
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