Simultaneous determination of multiple mRNA levels utilizing MALDI-TOF mass spectrometry and biotinylated dideoxynucleotides

Here we report an efficient method to simultaneously measure multiple mRNA levels utilizing mass spectrometry (MS) and molecular affinity isolation. In this approach, reverse transcription products of a group of mRNAs are subjected to competitive PCR with competitors and internal standards of known...

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Bibliographic Details
Main Authors: Duffield, Daniel Scott, Cai, Li, Kim, Sobin
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2010
Subjects:
Method
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2874180/
https://ncbi.nlm.nih.gov/pubmed/20410241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1859810
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