Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage

Real-time reverse transcription PCR (RT-qPCR) relies on a housekeeping or normalizer gene whose expression remains constant throughout the experiment. RT-qPCR is commonly used for characterization of human bone marrow mesenchymal stem cells (hBMSCs). However, to the best of our knowledge, there are...

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Autors principals: Quiroz, Felipe Garcia, Posada, Olga M., Gallego-Perez, Daniel, Higuita-Castro, Natalia, Sarassa, Carlos, Hansford, Derek J., Agudelo-Florez, Piedad, López, Luis E.
Format: Artigo
Idioma:Inglês
Publicat: Springer Netherlands 2010
Matèries:
Original Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2873986/
https://ncbi.nlm.nih.gov/pubmed/20396946
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-010-9265-1
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