Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR

BACKGROUND: The detection of Brucellae in tissue specimens using PCR assays is difficult because the amount of bacteria is usually low. Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA....

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Bibliographic Details
Main Authors: Tomaso, Herbert, Kattar, Mireille, Eickhoff, Meike, Wernery, Ulrich, Al Dahouk, Sascha, Straube, Eberhard, Neubauer, Heinrich, Scholz, Holger C
Format: Artigo
Language:Inglês
Published: BioMed Central 2010
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2873554/
https://ncbi.nlm.nih.gov/pubmed/20403208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2334-10-100
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