Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

BACKGROUND: Trigonopsis variabilis D-amino acid oxidase (TvDAO) is a well characterized enzyme used for cephalosporin C conversion on industrial scale. However, the demands on the enzyme with respect to activity, operational stability and costs also vary with the field of application. Processes that...

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Detalhes bibliográficos
Main Authors: Abad, Sandra, Nahalka, Jozef, Bergler, Gabriele, Arnold, S Alison, Speight, Robert, Fotheringham, Ian, Nidetzky, Bernd, Glieder, Anton
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
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Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2873405/
https://ncbi.nlm.nih.gov/pubmed/20420682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-9-24
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