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Protein Thermostability Calculations Using Alchemical Free Energy Simulations

Thermal stability of proteins is crucial for both biotechnological and therapeutic applications. Rational protein engineering therefore frequently aims at increasing thermal stability by introducing stabilizing mutations. The accurate prediction of the thermodynamic consequences caused by mutations,...

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Autores principales: Seeliger, Daniel, de Groot, Bert L.
Formato: Artigo
Lenguaje:Inglês
Publicado: The Biophysical Society 2010
Materias:
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2872215/
https://ncbi.nlm.nih.gov/pubmed/20483340
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.01.051
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