BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals

The interpretation of genome sequences requires reliable and standardized methods to assess protein function at high throughput. Here we describe a fast and reliable pipeline to study protein function in mammalian cells based on protein tagging in bacterial artificial chromosomes (BACs). The large s...

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Main Authors: Poser, Ina, Sarov, Mihail, Hutchins, James R A, Hériché, Jean-Karim, Toyoda, Yusuke, Pozniakovsky, Andrei, Weigl, Daniela, Nitzsche, Anja, Hegemann, Björn, Bird, Alexander W, Pelletier, Laurence, Kittler, Ralf, Hua, Sujun, Naumann, Ronald, Augsburg, Martina, Sykora, Martina M, Hofemeister, Helmut, Zhang, Youming, Nasmyth, Kim, White, Kevin P, Dietzel, Steffen, Mechtler, Karl, Durbin, Richard, Stewart, A Francis, Peters, Jan-Michael, Buchholz, Frank, Hyman, Anthony A
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2871289/
https://ncbi.nlm.nih.gov/pubmed/18391959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1199
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