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Efficient Electrotransformation of Bacteroides fragilis

This study describes refined electroporation parameters for efficient transformation of Bacteroides fragilis by plasmids prepared from laboratory strains of Escherichia coli. Development of the method used included determination of the optimal growth conditions for competent cell preparation, select...

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Bibliografiske detaljer
Main Authors: Ichimura, Minoru, Nakayama-Imaohji, Haruyuki, Wakimoto, Shin, Morita, Hidetoshi, Hayashi, Tetsuya, Kuwahara, Tomomi
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2869128/
https://ncbi.nlm.nih.gov/pubmed/20348295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02420-09
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