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Expression in Escherichia coli of the Native cyt1Aa from Bacillus thuringiensis subsp. israelensis

The gene cyt1Aa is one of the genes in the complex determining the mosquito larvicidity of Bacillus thuringiensis subsp. israelensis. Previous cloning in Escherichia coli resulted in a 48-bp addition upstream, encoding a chimera. Here, cyt1Aa was recloned without the artifact, and its toxicity again...

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Hlavní autoři: Sazhenskiy, Vladislav, Zaritsky, Arieh, Itsko, Mark
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2869120/
https://ncbi.nlm.nih.gov/pubmed/20348307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03068-09
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