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Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography.

We recently found that many RNA polymerase II transcription factors are modified with N-acetylglucosamine residues. These sugar moieties confer upon transcription factors an ability to bind the lectin wheat germ agglutinin. We have taken advantage of this interaction to devise a purification procedu...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Jackson, S P, Tjian, R
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286788/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2648381/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.6.1781
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