Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography.
We recently found that many RNA polymerase II transcription factors are modified with N-acetylglucosamine residues. These sugar moieties confer upon transcription factors an ability to bind the lectin wheat germ agglutinin. We have taken advantage of this interaction to devise a purification procedu...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1989
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286788/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2648381/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.6.1781 |
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