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Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.
The KEX2-encoded endoprotease was overproduced in yeast several hundred-fold and further purified to achieve a 10,000-fold enrichment in specific activity. The enzyme was (i) membrane-bound, but solubilized by detergents; (ii) able to cleave peptide substrates at both Lys-Arg and Arg-Arg sites; (iii...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286710/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2646633/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.5.1434 |
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