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Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical

To determine the radiation sensitivity of galactose oxidase, a 68 kDa monomeric enzyme containing a mononuclear copper ion coordinated with an unusually stable cysteinyl-tyrosine (Cys-Tyr) protein free radical. Both active enzyme and reversibly rendered inactive enzyme were irradiated in the frozen...

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Detalhes bibliográficos
Main Authors: Kempner, Ellis S, Whittaker, James W, Miller, Jay H
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2865722/
https://ncbi.nlm.nih.gov/pubmed/19998406
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.302
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