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Expression of human ferredoxin and assembly of the [2Fe-2S] center in Escherichia coli.

A cDNA fragment encoding human ferredoxin, a mitochondrial [2Fe-2S] protein, was introduced into Escherichia coli by using an expression vector based on the approach of Nagai and Thøgersen [Nagai, K. & Thøgersen, M. C. (1984) Nature (London) 309, 810-812]. Expression was under control of the lambda...

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Publicat a:Proc Natl Acad Sci U S A
Autors principals: Coghlan, V M, Vickery, L E
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1989
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Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC286572/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2644647/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.3.835
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