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Global and unbiased detection of splice junctions from RNA-seq data

We have developed a new strategy for de novo prediction of splice junctions in short-read RNA-seq data, suitable for detection of novel splicing events and chimeric transcripts. When tested on mouse RNA-seq data, >31,000 splice events were predicted, of which 88% bridged between two regions separ...

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Bibliografiske detaljer
Main Authors: Ameur, Adam, Wetterbom, Anna, Feuk, Lars, Gyllensten, Ulf
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864574/
https://ncbi.nlm.nih.gov/pubmed/20236510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2010-11-3-r34
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