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MISSA Is a Highly Efficient in Vivo DNA Assembly Method for Plant Multiple-Gene Transformation(1)([C])([W])

We describe a highly efficient in vivo DNA assembly method, multiple-round in vivo site-specific assembly (MISSA), which facilitates plant multiple-gene transformation. MISSA is based on conjugational transfer, which is driven by donor strains, and two in vivo site-specific recombination events, whi...

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Detalhes bibliográficos
Main Authors: Chen, Qi-Jun, Xie, Min, Ma, Xiao-Xiao, Dong, Li, Chen, Jia, Wang, Xue-Chen
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Biologists 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2862421/
https://ncbi.nlm.nih.gov/pubmed/20200068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.109.152249
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