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NMR Structure and Dynamics of the Engineered Fluorescein-Binding Lipocalin FluA Reveals Rigidification of β-Barrel and Variable Loops upon Enthalpy-Driven Ligand Binding

The NMR structure of the 21 kDa lipocalin FluA, which was previously obtained by combinatorial design, elucidates a reshaped binding site specific for the dye fluorescein resulting from 21 side chain replacements with respect to the parental lipocalin, the naturally occurring bilin-binding protein (...

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書目詳細資料
Main Authors: Mills, Jeffrey L., Liu, Gaohua, Skerra, Arne, Szyperski, Thomas
格式: Artigo
語言:Inglês
出版: 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2860743/
https://ncbi.nlm.nih.gov/pubmed/19603796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900535j
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