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Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain
Multi-polypeptide proteins such as antibodies are difficult to express in prokaryotic systems such as E. coli due to the complexity of protein folding plus secretion. Thus far, proprietary strains or fermenter cultures have been required for appreciable yields. Previous studies have shown that expre...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2857744/ https://ncbi.nlm.nih.gov/pubmed/20422027 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0010261 |
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