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Assay and regulation of S-adenosylmethionine synthetase in Saccharomyces cerevisiae and Candida utilis.

A simple and sensitive assay for S-adenosylmethionine (SAM) synthetase is described which depends on the quantitative separation of the product, [14CH3]S-adenosylmethionine, from the substrate, L-[14CH3]methionine, on a Bio-Rex 70 column. L-Methionine protects the enzyme during preparation of cell e...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Holcomb, E R, Shapiro, S K
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1975
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC285640/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1090572/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.121.1.267-271.1975
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