Synchronous germination and outgrowth of fractionated Bacillus subtilis spores: tool for the analysis of differentiation and division of bacterial cells.

A fraction of Bacillus subtilis 168 spores, purified by Urografin isopycnic density centrifugation, heat activated, and inoculated in nutrient broth plus glucose, germinated and outgrew very synchronously. Synchrony was documented by nuclear staining and fluorescence microscopy, and by determining t...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Siccardi, A G, Galizzi, A, Mazza, G, Clivio, A, Albertini, A M
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1975
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC285607/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/803948/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.121.1.13-19.1975
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