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Inhibition of the Bacillus subtilis Membrane-Bound d-Alanine Carboxypeptidase by 6-Aminopenicillanic Acid Covalently Coupled to Sepharose

An insoluble penicillin derivative, 6-aminopenicillanic acid covalently coupled to Sepharose, was used to investigate the location of the d-alanine carboxypeptidase in the Bacillus subtilis membrane. Only 50% of the enzymatic activity in protoplasts and 74% of that in purified membranes was sensitiv...

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Bibliographic Details
Main Authors: Storm, Daniel R., Blumberg, Peter M., Strominger, Jack L.
Format: Artigo
Language:Inglês
Published: 1974
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC285573/
https://ncbi.nlm.nih.gov/pubmed/4204441
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