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Comparison of polyclonal expansion methods to improve the recovery of cervical cytobrush-derived T cells from the female genital tract of HIV-infected women

Cervical cytobrushing is a useful and non-invasive method for obtaining mucosal mononuclear cells from the female genital tract, but yields few cells. The aim of this study was to compare in vitro expansion protocols (anti-CD3, anti-CD3/CD28 or Dynal anti-CD3/CD28 beads) and cytokine combinations (I...

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Detalhes bibliográficos
Main Authors: Bere, Alfred, Denny, Lynette, Hanekom, Willem, Burgers, Wendy A., Passmore, Jo-Ann S.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2854893/
https://ncbi.nlm.nih.gov/pubmed/20149794
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2010.02.002
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