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Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysis

This protocol describes imaging and computational tools to collect and analyze live imaging data of embryonic cell migration. Our five step protocol requires a few weeks to move through embryo preparation and four-dimensional (4D) live imaging using multiphoton microscopy, to 3D cell-tracking using...

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Autors principals: Supatto, Willy, McMahon, Amy, Fraser, Scott E., Stathopoulos, Angelike
Format: Artigo
Idioma:Inglês
Publicat: 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2854020/
https://ncbi.nlm.nih.gov/pubmed/19745822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.130
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