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Quantitative imaging of collective cell migration during Drosophila gastrulation: multiphoton microscopy and computational analysis
This protocol describes imaging and computational tools to collect and analyze live imaging data of embryonic cell migration. Our five step protocol requires a few weeks to move through embryo preparation and four-dimensional (4D) live imaging using multiphoton microscopy, to 3D cell-tracking using...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2854020/ https://ncbi.nlm.nih.gov/pubmed/19745822 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.130 |
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