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Sample complexity reduction for two-dimensional electrophoresis using solution isoelectric focusing prefractionation

Despite its excellent resolving power, 2-DE is of limited use when analyzing cellular proteomes, especially in differential expression studies. Frequently, fewer than 2000 protein spots are detected on a single 2-D gel (a fraction of the total proteome) regardless of the gel platform, sample, or det...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Richardson, Matthew R., Liu, Sean, Ringham, Heather N., Chan, Victor, Witzmann, Frank A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2853967/
https://ncbi.nlm.nih.gov/pubmed/18494027
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200700707
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