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Glycosylases and AP-cleaving enzymes as a general tool for probe-directed cleavage of ssDNA targets
The current arsenal of molecular tools for site-directed cleavage of single-stranded DNA (ssDNA) is limited. Here, we describe a method for targeted DNA cleavage that requires only the presence of an A nucleotide at the target position. The procedure involves hybridization of a complementary oligonu...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2853139/ https://ncbi.nlm.nih.gov/pubmed/20081204 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1238 |
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