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Rapid Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis by High-Resolution Melting Analysis

We have developed a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis. For assay development, 23 drug-resistant isolates of M. tuberculosis hav...

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Detalhes bibliográficos
Main Authors: Ong, Danny C. T., Yam, Wing-Cheong, Siu, Gilman K. H., Lee, Ann S. G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849564/
https://ncbi.nlm.nih.gov/pubmed/20164280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02036-09
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