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High-Throughput Method for Rapid Induction of Prophages from Lysogens and Its Application in the Study of Shiga Toxin-Encoding Escherichia coli Strains

A high-throughput 96-well plate-based method for the rapid induction of endogenous prophages from individual bacterial strains was developed. The detection of endogenous prophages was achieved by the filtration of the culture liquor following norfloxacin induction and subsequent PCRs targeting bacte...

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Detalhes bibliográficos
Main Authors: McDonald, James E., Smith, Darren L., Fogg, Paul C. M., McCarthy, Alan J., Allison, Heather E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849228/
https://ncbi.nlm.nih.gov/pubmed/20139312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02923-09
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