Coupling Molecular Beacons to Barcoded Metal Nanowires for Multiplexed, Sealed Chamber DNA Bioassays

We have combined molecular beacon (MB) probes with barcoded metal nanowires to enable no-wash, sealed chamber, multiplexed detection of nucleic acids. Probe design and experimental parameters important in nanowire-based MB assays are discussed. Loop regions of 24 bases and 5 base pair stem regions i...

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Main Authors: Stoermer, Rebecca L., Cederquist, Kristin B., McFarland, Sean K., Sha, Michael Y., Penn, Sharron G., Keating, Christine D.
Format: Artigo
Sprog:Inglês
Udgivet: 2006
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849162/
https://ncbi.nlm.nih.gov/pubmed/17177440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja0658261
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