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Total Synthesis of Acetate from CO(2) II. Purification and Properties of Formyltetrahydrofolate Synthetase from Clostridium thermoaceticum

Formyltetrahydrofolate synthetase from Clostridium thermoaceticum had optimal activity at 55 to 60 C. K(m) values were: adenosine triphosphate (ATP), 6.25 × 10(−4)m; formate, 1.67 × 10(−3)m; and tetrahydrofolate, 1.00 × 10(−3)m; at pH 8.0. There was a requirement for divalent metals and specifically...

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Detalhes bibliográficos
Main Authors: Sun, Albert Y., Ljungdahl, Lars, Wood, Harland G.
Formato: Artigo
Idioma:Inglês
Publicado em: 1969
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC284899/
https://ncbi.nlm.nih.gov/pubmed/5784233
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